International Standard
ISO/TS 19006:2016
Nanotechnologies — 5-(and 6)-Chloromethyl-2’,7’ Dichloro-dihydrofluorescein diacetate (CM-H2DCF-DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line
Reference number
ISO/TS 19006:2016
ISO/TS 19006:2016
Edition 1
2016-08
2016-08
© ISO 2024
International Standard
ISO/TS 19006:2016
63697
Published
(Edition 1, 2016)
This standard was last reviewed and confirmed in 2023.
Therefore this version remains current.
Abstract
ISO/TS 19006:2016 describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H2DCFDA assay.
The protocol in ISO/TS 19006:2016 is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.
General information
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Status: PublishedPublication date: 2016-08Stage: International Standard confirmed [90.93]
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Edition: 1Number of pages: 21
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Technical Committee :ISO/TC 229ICS :07.120
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